DNA extraction, amplification by PCR and purification for identification using sequencing techniques

 

Laboratory equipment that will be available:

SimpliAmp™ Thermal Cycler (PCR), pipettes, etc.

 

Laboratory to which access is provided:

Laboratory of Biochemistry, Chemistry Department, University of Ioannina

 

Methods that are used in the laboratory:

In-house methods for DNA extraction from plant tissues, amplification of selected regions by polymerase chain reaction (PCR) and purification of the products for subsequent identification by DNA sequencing.

 

Permitted time for access to the laboratory:

Access to equipment and lab areas is provided during business hours for a maximum of 5 days per user.

 

Time period for access to the service:

1 June – 30 June 2023

 

Information – Clarifications:

In the infrastructure of the University of Ioannina you will find specialized staff as well as all the necessary equipment with the consumables you will need for DNA extraction, amplification of selected regions by polymerase chain reaction (PCR) and purification so that they are ready for identification with the DNA sequencing technique. You can use existing methodologies used in the lab or one of your own. All the necessary supplies will be available for you to complete the process. If a specific genome region is requested for amplification, for which the laboratory does not have a primer set, it should be provided by you. This service can be combined with the sequencing data analysis service for the purpose of identification (DNA Sequencing – Identification) provided by the infrastructure. You will be able to process up to three (3) different matrices (species), from which you will receive the purified isolated DNA, ready for identification by sequencing.