Whole genome sequencing (WGS) of microorganisms

Scope description:

Next generation whole genome sequencing (WGS) of bacterial and fungal strains.

Laboratory to which access is provided:

Laboratory of Developmental Biology & Molecular Neurobiology, Department of Molecular Biology and Genetics, Democritus University of Thrace.

Laboratory equipment that will be available:

ION Torrent S5 sequencer, ION Chef system for automated library preparation, ION Touch 2 for manual library preparation, Qubit/Nanodrop, qPCR, dqPCR, -80°C deep freezer, computer systems / server and all other laboratory equipment required. 

Methods that are used in the laboratory:

In house methods for DNA isolation, library preparation, NGS sequencing and pipelines of analysis.

Permitted time to access the laboratory:

Access to equipment and lab facilities is provided during working hours for a maximum of 7 days per user.

Time period for access to the service:

1 June – 30 July and 1 September – 20 September 2023

Information – Clarifications:

In the Laboratory of Developmental Biology & Molecular Neurobiology of the Department of Molecular Biology and Genetics you will find well-trained staff, as well as all the necessary equipment you will need to perform whole genome sequencing of a microorganism. You will be able to bring your microbial culture and our research team will help you with all the steps of our in house methodology : genomic DNA isolation, DNA quantitation, library preparation, library quantitation and genome sequencing and bioinformatic analysis of data. At the end of the process, you will receive the raw data and the results of the analysis. All necessary reagents and materials will be available to enable you to complete the procedure. You are allowed to sequence one sample.